文獻名： Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles

作者： Alexander J. Wilson¹, Qingyu Zhou², Ian Vargas¹, Rohun Palekar³, Ryan Grabau¹, Hua Pan¹, Samuel A. Wickline¹

¹.The USF Health Heart InstituteUniversity of South FloridaTampaUSA

².College of PharmacyUniversity of South FloridaTampaUSA

³.Department of Biomedical EngineeringWashington University in St. LouisSt. LouisUSA

摘要：Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.

關鍵詞：Perfluorocarbon nanoparticles; Thrombin; PPACK; Bivalirudin; Anticoagulation